According to the manufacturer this assay is ten fold more sensitive than absorbance at 280 nm. The micro assay has a linear range of 15 100 µg ml using a 1 1 sample to reagent ratio. The coomassie brilliant blue protein assay commonly known as the bradford assay 1 is widely used because of its rapid and convenient protocol as well as its relative sensitivity unfortunately there is a large degree of curvature over a broad range of protein concentrations fig.
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